Wednesday, January 21, 2015

Antibody structure and Fragments

This is an educational video that shows the antibody structure and fragments that are made during digestion with various enzymes such as Pepsin, Papain and Ficin. If you need to conjugated your antibody through available free sulfur to the reactive group on the surface of the liposomes such as Maleimide, PDP, MCC or MPB then the antibody has to be digested and the disulfide bridge should be broken by using reducing agents such as DTT.

The video also shows various methods for making antibody fragments that are suitable for conjugation through N-terminus or C-terminus.