Immunoliposomes: Different strategies for conjugating antibodies to liposomes

Over the past two decades various techniques have been developed for conjugation of antibodies and peptides to the surface of liposomes. In this article we will be briefly describe some of these methods. 


 Methods that involve using a modified antibody


1) Attachment of an antibody to liposomes using a modified antibody (biotinylated antibody) and biotinylated DOPE lipid through avidin-biotin reaction

2) Attachment of an antibody to a liposome using a modified antibody (through an oxidized sugar on the Fc region) and Hydrazide-PEG-PE lipid

3) Attachment of an antibody to a liposome using either a thiolated antibody or a reduced antibody (Fab') using a MPB-PE lipid

4) Attachment of an MPB modified antbody to the liposomes using PDP-PE (DOPE or DSPE) lipid

5) Attachment of an antibody to a liposome using either a thiolated antibody or a reduced antibody (Fab') using liposomes containing NHSIA attached to DOPE

6) Attachment of an antibody to a liposome using either a thiolated antibody or a reduced antibody (Fab') using liposomes containing reactive lipid maleimide

7) Attachment of an antibody to a liposome using either a thiolated antibody or a reduced antibody (Fab') using MPB-PE liposomes 

8) Attachment of modified liposomes (by NHSIA) to modified antibodies (by SATA)

9) Attachment of an antibody to a liposome using either a thiolated antibody or a reduced antibody (Fab') using PDP-PE liposomes 

10) Attachment of liposomes (reacted with SPDP) to antibodies (reacted with SPDP and DTT)

Methods that involve using a non-modified antibody


1) Attachment of antibodies to liposomes containing P-NP group

2) Attachment of a reactive liposome containing carboxyl group to -NH2 group in antibody using EDC/Sulfo-NHS chemistry

3) Attachment of a reactive liposome containing amine group (PE) to -COOH group in antibody using EDC/Sulfo-NHS chemistry

This question was also answered on Quora:

http://www.quora.com/What-methods-are-used-for-conjugation-of-antibodies-to-liposomes?q=liposome