Wednesday, September 28, 2011

Immunoliposomes: Different strategies for conjugating antibodies to liposomes

Over the past two decades various techniques have been developed for conjugation of antibodies and peptides to the surface of liposomes. In this article we will briefly describe some of these methods. (Click on the images to magnify them)




 Conjugation through Modified Antibody

1) Biotin modified antibody





2) Thiol modified antibody



Conjugation of a thiol modified antibody to a PDP modified PEGylated lipid



Conjugation of a thiol modified antibody to a maleimide modified PEGylated lipid


Conjugation of a thiol modified antibody to an MBP modified lipid

Conjugation of a thiol modified antibody to an MCC modified lipid

3) Maleimide modified antibody


Conjugation of maleimide modified antibody to PDP modified PEGylated lipid

4) Aldehyde modified antibody


Conjugation of aldehyde modified antibody to a hydrazide modified lipid

5) Hydrazide modified antibody

Conjugation of hydrazide modified antibody to a aldehyde modified lipid

 Conjugation through N on Antibody

1) Carboxylic Acid Chemistry


Conjugation of EDC/NHS activated PEGylated carboxylic acid modified lipid to N-terminus of antibody

Conjugation of EDC/NHS activated PEGylated succinyl modified lipid to N-terminus of antibody

Conjugation of EDC/NHS activated succinyl modified lipid to N-terminus of antibody (non PEGylated)

Conjugation of EDC/NHS activated glutaryl modified lipid to N-terminus of antibody (non PEGylated)



Conjugation of EDC/NHS activated dodecanoyl modified lipid to N-terminus of antibody (non PEGylated)

2) NHS ester lipid


Conjugation of NHS ester lipid to N-terminus of antibody


3) Cyanur Chemistry



Conjugation of cyanur modified PEGylated lipid to N-terminus of antibody


Conjugation of cyanur modified lipid to N-terminus of antibody (non PEGylated)

Conjugation of cyanur caproyl modified lipid to N-terminus of antibody (non PEGylated)


 Conjugation through C on Antibody



Conjugation of amine modified PEGylated lipid to EDC/NHS activated antibody



Conjugation of phosphatidylethanolamine (PE) lipid to EDC/NHS activated antibody

Conjugation of caproylamine lipid to EDC/NHS activated antibody

Conjugation through S on Fragmented Antibody



Conjugation of maleimide modified PEGylated lipid to Fab' fragment


Conjugation of PDP modified PEGylated lipid to Fab' fragment


Conjugation of MBP modified  lipid to Fab' fragment (non-PEGylated)

Conjugation of PDP modified lipid to Fab' fragment (non-PEGylated)


Conjugation of MCC modified  lipid to Fab' fragment (non-PEGylated)

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